Gram positive

Gram positive:-

1. In these gram positive bacteria peptidoglycan is about 25 nano meter wide and contains and additional polysaccaharide called teichoic acid.
2. About 60-90% of cell wall is peptidoglycan.

The gram positive organism retain thre crystal violet after decolorized they appear violet in color.

 In general, gram-positive bacteria are monoderms and have a single lipid bilayer whereas gram-negative bacteria are diderms and have two bilayers.

 Some taxa lack peptidoglycan (such as the domain Archaea, the class Mollicutes, some members of the Rickettsiales, and the insect-endosymbionts of the Enterobacteriales) and are gram-variable.

 This, however, does not always hold true. The Deinococcus-Thermus bacteria have gram-positive stains, although they are structurally similar to gram-negative bacteria with two layers. 

The Chloroflexi have a single layer, yet (with some exceptions) stain negative.Two related phyla to the Chloroflexi, the TM7 clade and the Ktedonobacteria, are also monoderms